Principles of Cloning, Second Edition

Principles of Cloning, Second Edition

Jose Cibelli, Robert Lanza

Language: English

Pages: 572

ISBN: 0123865417

Format: PDF / Kindle (mobi) / ePub


  Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species―including mice, sheep, cattle, and non-mammals―is considered as well.   The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas.

  • First and most comprehensive book on animal cloning, 100% revised
  • Describes an in-depth analysis of current limitations of the technology and research areas to explore
  • Offers cloning applications on basic biology, agriculture, biotechnology, and medicine

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Transferred (%) Postnatal Death/No. of ES Cell-Derived Mice (%) 1011 26(3) 10/15(67) 1037 48(5) 21/34(62) 1976 20(1) 17/20(85) a ES cells were injected into blastocysts treated at 45°C for 20 min. ES cells were injected into tetraploid blastocysts. P < 0.05. b a-b TABLE 10.5  Effects of Cell Lines on the Developmental Potential of Somatic Cell Nuclear-Transferred Embryos Line 1 Origin  Total Recipients Pregnancies Abortions Calves Peri–or Postnatal Death (%) 20 10 5

1992. Control of the cell cycle. Dev. Biol. 153, 1–15. Iannaccone, P., Taborn, G., Garton, R., 2001. Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts. Zygote 9, 135–143. Ito, J., Hirabayashi, M., Kato, M., Takeuchi, A., Ito, M., Shimada, M., et al., 2005. Contribution of high p34cdc2 kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes. Reproduction 129, 171–180. Ito, J., Shimada, M., Hochi,

success of cloning experiments is due to having good manipulation tools. The two largest suppliers for pre-made micro­ manipulation tools are Cook Veterinary Products USA (Bloomington, IN) and Humagen Fertility Diagnostics Inc. (Charlottesville, VA). Both manufacturers offer custom services, so it is possible to obtain the unique tools you desire from commercial sources. However, once mastered, custom tool-making does not take too much time and allows the investigator to tailor the tools on the

outside the incubator is achieved. Many investigators opt for standard media developed in the industry, such as M2 (Hogan et  al., 1994) originally developed for mouse embryos, or TL–Hepes (Parrish et  al., 1986) originally developed for bovine embryos. These are relatively simply media requiring few components, and are easy to make. Due to the widespread applicability and use of KSOM (Lawitts and Biggers, 1991) and recent modifications (Biggers et al., 2000), commercial sources of KSOM and a

Lane, M., 2001. Mechanisms for managing cellular and homeostatic stress in vitro. Theriogenology 55, 225–236. Lane, M., Gardner, D.K., 1992. Effect of incubation volume and embryo density on the development and viability of mouse embryos in vitro. Hum. Reprod. 7, 558–562. Lane, M., Gardner, D.K., 1994. Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions. J. Reprod. Fertil. 102, 305–312. Lane, M.,

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